Research Article
Rajendra Singh Chauhan1*,
Dinesh Tewari1,2, Bhagwati Prasad Nautiyal1, Virendra
Singh Rana3 and Aldo
Tava4
Abstract
Valeriana wallichii DC. (Valerianaceae)
is an important medicinal
and aromatic plant species (MAPs) used in traditional, Ayurvedic, Unani and
modern system of medicines. Valerian
rhizomes used in herbal tea formulations for sleep aid due to sedative
properties. Volatile oil used in perfumery, flavour, pharmaceutical and
fragrance industries and insect repellent formulations. Over
utilization threatened its status in
natural habitat and sustainable use of species is recommended. Considering
increased demand, experimental cultivation initiated at Bharsar, Uttarakhand
(1900 m asl). Samples were collected from experimental cultivation trials (for
yield) during the month of March 2022. Essential
oil was extracted from freshly
harvested samples using a Clevenger type apparatus and analyzed by GC/FID and
GC/MS. Essential oil content was 0.46%
on fresh weight basis. Patchouli
alcohol
(39.78%) was the major constituent of the essential oil and other important
constituents were α-guaiene
(9.29%), α-bulnesene (6.96%),
seychellene (5.1%), α-patchoulene (3.29%), isovaleric
acid (2.41%), α-santalene (1.90%),
camphene 1.82%), etc. Variations
in the composition of the essential oil may be attributed to factors
related to the environment including temperature, relative humidity and
photoperiod at cultivation sites along with genetic makeup of the species.
This study opens new areas for cultivation, pharmacological and biological
activities for the studied germplasm.
Abstract Keywords
Essential oil, Indian System of
Medicine (ISM), MAPs, threatened plants, rhizomes, Valerianaceae
Abstract
Valeriana wallichii DC. (Valerianaceae) is an important medicinal and
aromatic plant species (MAPs) used in traditional, Ayurvedic, Unani and modern
system of medicines. Valerian rhizomes used in herbal tea
formulations for sleep aid due to sedative properties. Volatile oil used in
perfumery, flavour, pharmaceutical and fragrance industries and insect
repellent formulations. Over
utilization threatened its status in natural
habitat and sustainable use of species is recommended. Considering increased
demand, experimental cultivation initiated at Bharsar, Uttarakhand (1900 m
asl). Samples were collected from experimental cultivation trials (for yield) during
the month of March 2022. Essential
oil was extracted from freshly
harvested samples using a Clevenger type apparatus and analyzed by GC/FID and GC/MS. Essential oil content was 0.46% on fresh
weight basis. Patchouli alcohol (39.78%) was
the major constituent of the essential oil and other important constituents
were α-guaiene (9.29%), α-bulnesene (6.96%),
seychellene (5.1%), α-patchoulene (3.29%), isovaleric
acid (2.41%), α-santalene (1.90%),
camphene 1.82%), etc. Variations in
the composition of the essential oil may be attributed to factors
related to the environment including temperature, relative humidity and
photoperiod at cultivation sites along with genetic makeup of the species.
This study opens new areas for cultivation, pharmacological and biological
activities for the studied germplasm.
Keywords
Essential oil, Indian System of
Medicine (ISM), MAPs, threatened plants, rhizomes, Valerianaceae
References
1.
Bhattacharjee, S.K. Hand book of Aromatic
Plants. Pointer Publisher, Jaipur, India. 2000, p.458
2.
Prakash, V. Indian Valerianaceae: A Monograph on
Medicinally Important Family. Scientific Publishers, Jodhpur, India.1999.
3.
Atal, C.K.
and Kapur, B.M. Cultivation and Utilization of Medicinal and Aromatic Plants.
Regional Research Laboratory, Jammu‑Tawi. 1977, p.393
4.
Balderer, G. and Borbely, A. Effect of valerian on
human sleep. Psychopharmacology.
1985, 87, 406-409.
5.
Morazzoni, P. and Bombardelli, E. Valeriana
officinalis: traditional use and recent evaluation of activity. Fitoterapia. 1995, 66, 99-112.
6.
Chowdhury, A.R. GC/MS studies on the essential oil
from the roots of Valeriana wallichii DC. Indian Perfumer. 1999, 43,147‑9.
7.
Singh, A.; Rahman, L.; Kukreja, A.K.; Yadav, A.;
Naqvi, A.A. and Khanuja, S.P.S. Seasonal effect on essential oils content and
quality of Valeriana wallichii DC.
cultivated in Uttarakhand hills (India). Indian Perfumer. 2008, 52, 49-52.
8.
Verma, R.S.; Padalia, R.C. and Chauhan, A. Assessment of similarities and dissimilarities in the essential oils of
patchouli and Indian Valerian. J Essential Oil Research. 2012, 24, 487‑491.
9.
Raina, A. and Negi, D.K.S. Essential Oil Composition
of Valeriana Jatamansi Jones from
Himalayan Regions of India. Indian J Pharmaceutical Science. 2015, 218-222.
10.
Kaundal, M.; Bhatt, V. and Kumar, R. Elevated CO2
and Temperature Effect on Essential Oil Content and Composition of Valeriana
jatamansi Jones. with Organic Manure Application in a Western Himalayan
Region. J Essential
Oil-Bearing Plants. 2018, 21 (4), 1041-1050.
11.
Mathela, C.S.; Chanotiya, C.S.; Sati, S.; Tiwari, M.
and Sammal, S.S. Chemical perspective of Tagar-An Ayurvedic Drug. Indian J
Traditional Knowledge. 2006, 5(4), 474-477.
12.
Bhatt, I.D.; Dauthal, P.; Rawat, S.; Gaira, K.S.; Jugran, A.;
Rawal, R.S. and Dhar, U. Characterization of essential oil composition,
phenolic content, and antioxidant properties in wild and planted individuals of
Valeriana jatamansi Jones. Scientia
Horticulturae. 2012, 136, 61-68.
13.
Rawat, S.; Jugran, A.K.; Bhatt, I.D.; Rawal, R.S.; Andola, H.C. and Dhar, U. Essential oil composition and antioxidant activity in Valeriana
jatamansi Jones: influence of seasons and growing sources. J Essential Oil
Research. 2017, 29(1), 101-107.
14.
Ved, D.K. and Goraya, G.S. Demand and supply of
medicinal plants in India. Bishan Singh Mahendra Pal Singh, Dehradun &
FRLTH, Bangalore, India. 2008.
15.
Chauhan, R.S.; Tiwari, D.; Bisht,
A.S. and Shukla, A. Ex situ
conservation of medicinal and aromatic plants in Bharsar, Uttarakhand, India.
Medicinal Plants. 2014, 6 (4), 282-292.
16.
Adams, R.P. Identification
of Essential Oil Components by Gas Chromatography/Mass Spectrometry, 4th
edition. Allured Publishing Corporation, Carol Stream, IL. 2007.
17.
NIST/EPA/NIH NIST/EPA/NIH
Mass Spectral Database, Version 2.1, PerkinElmer Instrument LLC, Copyright
2000.
18.
Chauhan, R.S.; Nautiyal, M.C.; Figueredo, G. and Rana,
V.S. Effect of
post-harvest drying methods on the essential oil composition of Nardostachys jatamansi DC. J Essential
Oil-Bearing Plants. 2017, 20(4), 1090-1096.
19.
Gunther, R.T. The Greek herbal of Discorides. Hafner, London. 1968, p.10
20.
Bos, R.; Woerdenbag, H. J.; Hendriks, H.; Smit, H. F.;
Wikström, H. V. and Scheffer, J. J. C. Composition of the essential oil from
roots and rhizomes of Valeriana wallichii DC. Flavour and Fragrance
Journal. 1997, 123 - 131.
21.
Saklani,
N.; Purohit, V.K.; Andola, H.C.; Chauhan, R.S. and Nautiyal, A.R. Biological
activities of Valeriana wallichii DC.
Medicinal Plants. 2012, 4 (3), 115-120.
22.
Jha, S. and Thakur, N.D. Efficacy
and Toxicity Study of Nardostachys Jatamansi DC and Valeriana
Wallichii DC. in sleep disorders. I.J. Res. Eng., Sci. and Management. 2020, 3(10), 100-106.
This work is licensed under the
Creative Commons Attribution
4.0
License (CC BY-NC 4.0).
Abstract
Valeriana wallichii DC. (Valerianaceae)
is an important medicinal
and aromatic plant species (MAPs) used in traditional, Ayurvedic, Unani and
modern system of medicines. Valerian
rhizomes used in herbal tea formulations for sleep aid due to sedative
properties. Volatile oil used in perfumery, flavour, pharmaceutical and
fragrance industries and insect repellent formulations. Over
utilization threatened its status in
natural habitat and sustainable use of species is recommended. Considering
increased demand, experimental cultivation initiated at Bharsar, Uttarakhand
(1900 m asl). Samples were collected from experimental cultivation trials (for
yield) during the month of March 2022. Essential
oil was extracted from freshly
harvested samples using a Clevenger type apparatus and analyzed by GC/FID and
GC/MS. Essential oil content was 0.46%
on fresh weight basis. Patchouli
alcohol
(39.78%) was the major constituent of the essential oil and other important
constituents were α-guaiene
(9.29%), α-bulnesene (6.96%),
seychellene (5.1%), α-patchoulene (3.29%), isovaleric
acid (2.41%), α-santalene (1.90%),
camphene 1.82%), etc. Variations
in the composition of the essential oil may be attributed to factors
related to the environment including temperature, relative humidity and
photoperiod at cultivation sites along with genetic makeup of the species.
This study opens new areas for cultivation, pharmacological and biological
activities for the studied germplasm.
Abstract Keywords
Essential oil, Indian System of
Medicine (ISM), MAPs, threatened plants, rhizomes, Valerianaceae
This work is licensed under the
Creative Commons Attribution
4.0
License (CC BY-NC 4.0).
Editor-in-Chief
Prof. Dr. Radosław Kowalski
This work is licensed under the
Creative Commons Attribution 4.0
License.(CC BY-NC 4.0).